After our PDMS microfluidic devices have been molded and cured they need to be bound to a glass coverslip (coverslips are used because they are thin. The focal point of our 100x oil immersion microscope objective would be inside a regular microscope slide). Our device and coverslip are placed in a vacuum chamber. Plasma is generated by radio frequency induction. The plasma cleans the surface of the glass and PDMS and makes them both hydrophilic. Once they have been plasma treated the PDMS is be irreversibly stuck to the glass.