I was in Ventura, California from January 8 - 13, 2017 to present a poster titled Resource Sharing Controls Gene Expression Bursting at the Stochastic Physics in Biology Gordon Research Conference: Landscapes, Stochastic Dynamics and Heterogeneity in Biology. The research is now published open access at ACS Synthetic Biology. The Gordon Conferences are unique. Everyone signs an NDA and no photos are allowed, so very new results are presented and discussed. The conference topics are very specific so the audience is small and mostly the world experts in the field. Because the conferences are small, discussion is encouraged from everyone, even grad students. Finally the conferences are in remote locations and there is an early afternoon break every day where people are encouraged to hang out. During this time I played soccer, tennis, biked and ran with professors, post docs, and other grad students. It was a great way to get to know people in a less academic, less intimidating way. I was very fortunate to meet Dr. Daniel Gillespie and his wife Carol Gillespie. He created the Gillespie algorithm which I use to make stochastic simulations of my experimental system. He spoke about how the algorithm was created and how he received great skepticism when he first presented it.
Bredesen Center for Interdisciplinary Research and Graduate Education
I am a PhD student at the University of Tennessee, Knoxville and Oak Ridge National Laboratory with a Bredesen Center Fellowship. I research noise in protein expression at under the guidance of my advisors Mike Simpson in the DOE sponsored Center for Nanophase Material Science and Mitch Doktycz in BioSciences Division.
Cell-sized, cell-free reactions are increasing in complexity and imitating many functions of living cells. They have membranes, the ability to make proteins, form pores, grow, metabolize glucose, and communicate with cells. However making protein, the basis for every living cell, is highly variable in these reactions. Identically prepared reactions often show order of magnitude differences in the amount of protein made chamber-to-chamber. Understanding the causes of this variability will allow greater functionality for cell-sized, cell-free reactions.
I became interested in biology and genetics after creating transgenic, green fluorescent bacteria in high school AP biology. As an undergraduate junior, I worked with Dr. Mingjun Zhang to model the Young’s modulus of a silicone matrix filled with dispersed nanoparticles. Senior year, I worked with Dr. Cong Trinh to create a collection of plasmids with different copy numbers and antibiotic resistances. These plasmids were then incorporated into other work being done in the lab for better control over enzyme production and the associated metabolic processes. I graduated with a Bachelor of Science in Biomedical Engineering with a minor in Engineering Entrepreneurship from the University of Tennessee, Knoxville.
My ORNL profile is here.
My Google Scholar profile is here.
Norred, S. Elizabeth, Patrick M. Caveney, Gaurav Chauhan, Lauren K. Collier, C. Patrick Collier, Steven M. Abel, and Michael L. Simpson. "Macromolecular Crowding Induces Spatial Correlations That Control Gene Expression Bursting Patterns." ACS synthetic biology 7, no. 5 (2018): 1251-1258.
Boreyko, Jonathan, P. Caveney, Sarah L. Norred, Charles Chin, Scott T. Retterer, Michael L. Simpson, and C. Pat Collier. "Synthetic Biology in Aqueous Compartments at the Micro-and Nanoscale." MRS Advances 2, no. 45 (2017): 2427-2433.
Caveney, Patrick M., S. Elizabeth Norred, Charles W. Chin, Jonathan B. Boreyko, Brandon S. Razooky, Scott T. Retterer, C. Patrick Collier, and Michael L. Simpson. "Resource Sharing Controls Gene Expression Bursting." ACS Synthetic Biology (2016). [PDF]
Norred, S. E., Caveney, P. M., Retterer, S. T., Boreyko, J. B., Fowlkes, J. D., Collier, C. P., et al. Sealable Femtoliter Chamber Arrays for Cell-free Biology. J. Vis. Exp. (97), e52616, doi:10.3791/52616 (2015).
Lijin Xia, Zhonghua Xu, Leming Sun, Patrick M. Caveney, Mingjun Zhang. (2013) “Nano-fillers to tune Young's modulus of silicone matrix.” Journal of Nanoparticle Research. v. 15 pg. 1570. [PDF]
Graduate Courses Taken
LFSC 507 - Programming for Biological Data Analysis
STAT 537 - Statistics for Research I
ESE 597 Bioenergy Journal Club
MSE 578 Advanced Biomaterials/Nanomaterials
PHYS 642 BioPhysics
ESE 697 Stochastic Processes Bio/Sys
CBE 691 Biomolecular Kinetics and Cellular Dynamics
MATH 527 Stochastic Modeling
ESE 593 Independent Study
ESE 593 Independent Study: Entrepreneurship
ESE 593 Entrepreneurship Studies
ESE 511 Introduction to Energy Science & Technology I
ESE 512 Introduction to Energy Science & Technology II